2 edition of study of enamel and root pellicles formed from parotid saliva in vitro found in the catalog.
study of enamel and root pellicles formed from parotid saliva in vitro
Stanwick Lam Watt
|Statement||by Stanwick Lam Watt.|
Understanding the composition and structure of the acquired enamel pellicle (AEP) has been a major goal in oral biology. Our lab has conducted studies on the composition of AEP formed on permanent enamel. The exhaustive exploration has provided a comprehensive identification of more than proteins from AEP formed on permanent enamel. The AEP formed on deciduous enamel has not Whole mouth saliva (WMS) is produced by the three main salivary glands: sublingual, submandibular and parotid, along with many minor salivary glands located throughout the oral mucosa. Saliva consists of over 99% water, the remainder containing a mixture of organic and inorganic substances (Engelen et al.
Oppenheim FG, Offner GD, Troxler RF. Phosphoproteins in the parotid saliva from the subhuman primate Macaca fascicularis. Isolation and characterization of a proline-rich phosphoglycoprotein and the complete covalent structure of a proline-rich phosphopeptide. J Biol Salivary secretion is under autonomic nervous control and thus regulated by the parasympathetic and sympathetic nervous system. Impulses that arise from activation of chemoreceptors in the taste buds are carried to the salivatory nuclei in the medulla oblongata through the trigeminal (V), facial (VII), glossopharyngeal (IX) and vagal (X) nerves (NN.), whereas impulses from activation of
The indigenous microbiota plays an important role in health and diseases of humans and animals. It contributes to the development of the immune system and provides resistance to colonization by allochthonous or pathogenic microorganisms (95, , , , ).It also constitutes a reservoir of potentially pathogenic bacteria that may infect host tissues (44, , ). 2 Saliva. The reflection of body status in saliva could potentially be used to monitor general health, disease onset and progression. Protein biomarkers in biological fluids in particular, which can be measured accurately and reproducibly, may provide valuable information on the body’s response to a treatment for a disease or condition, including the long-term monitoring of oral ://
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Lam Watt and Bennick () noted that salivary pellicles formed for 2 h in vitro on enamel contain much more APRP than the pellicle formed on root surfaces. We found another class of phos- phoproteins in saliva, cystatin S, which also exhibit this differential :// Atomic force microscopy study of salivary pellicles formed on enamel and glass in vivo Article (PDF Available) in Colloid and Polymer Science (5) May with Reads Effect of lipids on the lactic acid retardation capacity of tooth enamel and cementum pellicles formed in vitro from saliva of caries-resistant and caries-susceptible human adults Author links open overlay panel B.L.
Slomiany 1 V.L.N. Murty 1 I.D. Mandel 2 S. Sengupta 1 A. Slomiany 1 In this study we investigated the differences in the properties of pellicles formed from stimulated parotid saliva (PS), which contains little or no mucin; and stimulated whole mouth saliva (WMS In this study we investigated the differences in the properties of pellicles formed from stimulated parotid saliva (PS), which contains little or no mucin; and stimulated whole mouth saliva (WMS), which contains mainly two types of mucin: MUC5B and :// Pellicle forms on constituents of surface pellicle formed on human enamel and cementum werecompared under three conditions: (1) natural pellicle, present on extracted teeth, which was formed by prolonged exposure to human salivary and serum components in vivo; (2) short-term in-vivo pellicle, formed by exposing enamel and cementum slabs to the oral environment for 0–60 min; (3) in-vitro Introduction.
The salivary pellicle is a protein rich film that forms on all surfaces within the mouth, and provides a barrier to dissolution of enamel by dietary acids, and concomitantly lubricates the mouth facilitating the consumption and processing of food (Carpenter ).However, the pellicle is ambivalent in nature as it also provides the primary sites for the attachment of bacteria It is interesting to compare these results with those obtained in experiments on the in-vitro formation of new pellicle on roots and enamel from human teeth.
In these pellicles, proline- rich proteins account for per cent total extracted protein from the root surfaces, but per cent of the protein extracted from the enamel surfaces (Lam Wetton S, Hughes J, West N, Addy M.
exposure time of enamel and dentine to saliva for protection against erosion: a study in vitro. Caries Res. ; [ Links ] Wiegand A, Bliggenstorfer S, Magalhães AC, Sener B, Attin T. Impact of the in situ formed salivary pellicle on enamel and dentine erosion induced by different acids ?script=sci_arttext&pid=S Significantly less enamel and dentin loss could therefore be observed for samples stored in situ compared with storage in water or saliva in vitro Due to the different composition of dental hard tissues it may be assumed that the protective properties of the pellicle can vary for enamel II) BONDING AND SALIVA: A few studies are hereby mentioned to see the ever changing influence of saliva in bonding procedure with advent of new generation of bonding agents, primers.
1) Effect on a New Bonding agent in bond strength to saliva contaminated enamel: The purpose of the study in vitro was to compare bond strengths of brackets Abstract. The acquired enamel pellicle (AEP) is an organic film, bacteria-free, formed in vivo as a result of the selective adsorption of salivary proteins and glycoproteins to the solid surfaces exposed to the oral environment.
Objective: This study aimed to compare the proteomic profile of AEP formed in situ on human and bovine enamel using a new intraoral device (Bauru in situ pellicle ?script=sci_arttext&pid=S In vitro pellicles were formed as described in the literat28,  through the immersion of specimens in natural saliva over a specific time period.
After immersion Saliva collection and in vitro bacterial overlay. The salivary flow rates were ml/min (s.d. ml/min) for unstimulated saliva and ml/min (s.d. ml/min) for the gum-base The aim of the present study was to use an in vitro enamel demineralization model (1) to confirm that whole saliva pretreatment conferred acid resistance to dental enamel and (2) to determine The protein compositions of in vitro pellicles formed from whole saliva and parotid and submandibular secretions were determined by use of synthetic hydroxyapatite as a model for dental enamel Previous studies have shown greater adhesion of S.
gordonii to human parotid saliva (HPS)- and amylase-coated hydroxyapatite (HAP) compared to non-amylase-binding oral streptococci.
It has also been shown that incubation of S. gordonii in the presence of maltotriose increases adhesion to experimental pellicles formed on HAP.
A role for If access of saliva to the plaque is prevented there is a dramatic fall in plaque pH, whereas unrestricted salivary flow to plaque results in little alteration of plaque pH. Saliva is therefore able to prevent acidification of plaque. Resting parotid saliva has a pH of and bicarbonate conc.
of m Eq/L. Whereas at high flow rates the pH Results. Secretory IgA, α-amylase and cystatins were identified as dominant proteins in the salivary pellicles.
Selective adsorption of proteins was demonstrated by the enrichment of prolactin-inducible protein and absence of zinc-α 2-glycoprotein relative to nce of to titanium led to an up-regulation of metabolic activity in the population after 2 :// In vitro pellicles were formed as described in the literat 28, 33 – 39 through the immersion of specimens in natural saliva over a specific time period.
After immersion for 24 h in saliva, specimens were stored un-agitated overnight at 22 °C ±. Salivary pellicles on smooth titanium surfaces were desorbed and these, as well as purified human saliva, were subjected to two-dimensional gel electrophoresis and mass spectroscopy.
A parallel plate flow-cell model was used to study binding of a fresh isolate of S. oralis to uncoated and saliva-coated titanium :// Saliva is the most relevant biological factor for the prevention of dental erosion.
It starts acting even before the acid attack, with an increase of the salivary flow rate as a response to the acidic stimuli. This creates a more favorable scenario, improving the buffering system of saliva and effectively diluting and clearing acids that come in co New in vitro model for the acquired enamel pellicle: pellicles formed from whole saliva show inter-subject consistency in protein composition and proteolytic fragmentation patterns.
J Dent Res